A major challenge in modern biology is the discovery of in vivo metabolic or physiological functions of unknown proteins. Our institute has developed an integrated strategy based on in silico prediction of enzymatic activities and in vitro screening of enzymes
MetX and MetA are two phylogenetically unrelated protein families, but both are implied in the first step of the L-methionine biosynthesis pathway. MetX proteins are known as L-homoserine O-acetyltransferases (HAT), while MetA proteins are generally annotated as L-homoserine O-succinyltransferase (HST).
We developed a method to classify proteins of a family based on their conserved genomic contexts. Each protein genomic context is compared against all others to determine syntenies. A graph is generated where nodes are input proteins connected by edges
Nearly 35% of the proteins from large-scale sequencing of microbial genomes are annotated with unknown function. Our objective is to explore, by analogy, active sites from proteins of unknown function using 3D tools in order to suggest enzymatic activities. The
There is a massive amount of sequence and structural data available, and the accumulation rate exceeds the pace of functional studies. One way to enhance functional assessment is to mine the available data to inform a strategy that can be
The proportion of protein sequences of unknown function in public databases stills very important (42% of UniProt sequences are labelled as “hypothetical”, “uncharacterized”, “unknown” or “putative”). On the other hand, a number of enzyme activities (about 30%) remain orphan (i.e.
Contexte : Le Genoscope (CEA, Institut de Biologie François Jacob) est un centre de référence français en génomique environnementale qui réalise des analyses à grande échelle pour la compréhension des écosystèmes, notamment dans le contexte des grands changements environnementaux. Son
Analyse pangénomique des voies métaboliques chez les archées Description Lieu : Vous serez accueilli au sein du Laboratoire d’Analyses Bioinformatiques pour la Génomique et le Métabolisme (LABGeM, UMR8030, https://labgem.genoscope.cns.fr) du Genoscope (Institut de biologie François Jacob – CEA – Evry),