methodological development


Next-generation sequencing (NGS) technology has revolutionized genomics leading to an unrivaled generation of large overloads of omics data ((meta)genomes, (meta)transcriptomes, epigenomes). The use of sequencing machines, which are now smaller in size but capable of generating piles of data faster and at a lower cost, have however raised the challenges of sharing, archiving, integrating and analyzing these data. Efficient and effective pipelines/tools to deal with those increasing amounts of genomics data are therefore needed to store, transfer, analyze, visualize, and generate reports to researchers and possibly clinicians. In this scope, at LABGeM we develop standard as well as innovative tools to get the most of NGS data.


PanGBank: exploring GenBank genomes through a resource of pangenome graphs

Many comparative genomic studies try to get a grasp on the overall gene content of a species. However, with the current explosion of available genomic data, it becomes more complex to use all-vs-all genome comparison approaches. Over the last years, ...
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panRGP: a pangenome-based method to predict genomic islands and explore their diversity

Horizontal gene transfer (HGT) is a major source of variability in prokaryotic genomes. Regions of Genome Plasticity (RGPs) are clusters of genes located in highly variable genomic regions. Most of them arise from HGT and correspond to Genomic Islands (GIs) ...
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The MicroScope platform raised in the Cloud: toward a Software as a Service for on-demand analyses of microbial genomes (MicroCloud)

MicroScope is an integrated platform to support microbial genomes (re)annotation and comparative analysis. The current project aims at designing a version of the MicroScope platform using Cloud technologies to progressively switch into a Software as a Service (SaaS) distribution mode ...
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Conceptualization and exploitation of a partitioned pangenome graph as a compact representation dedicated to the characterization of the diversity of the genomic repertoire of a bacterial species

Introduced in microbiology in 2005 by Medini et al. and Tettelin et al., pangenome approaches aim to compile the entire genomic diversity of a species. In these studies, we generally distinguish within the pangenome, the core genome: the set of ...
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Functional Annotation

High quality functional annotation is essential for understanding the phenotypic consequences encoded in a genome. We develop several methods to improve protein function predictions. These methods are mainly based on sequence analysis, genomic and metabolic context exploration and structural bioinformatics.

Prediction, annotation and exploration of genomic regions in MicroScope

In order to extend the range of tools available to MicroScope users, we aim to develop the platform functionalities dedicated prediction, annotation and exploration of genomic regions. These genomic regions include specialized gene clusters (ie, encoding for secretion systems, CRISPR systems, Biosynthetic Gene Clusters, operons, etc.), phage or GI / ...
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NetSyn – Exploring Syntenies Networks of Genes

We developed a method to classify proteins of a family based on their conserved genomic contexts. Each protein genomic context is compared against all others to determine syntenies. A graph is generated where nodes are input proteins connected by edges when a synteny is observed. Edges weight represent the mean ...
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